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High Performance Liquid Chromatography (HPLC) instruments are used for the analysis of non-volatile or thermally stable compounds. It is one of the most widely used instrumentation for the analysis pharmaceutical products, isolation of natural products, petrochemical industry, and compounds of environmental interest, among others. This instrument is equipped with a diode array detector, which has the capability of measuring simultaneously wavelengths in the UV-Visible range, thus providing more information than traditional fixed wavelengths detectors. In order to be suitable for the analysis the sample must absorb radiation in the UV-Vis range (usually organic compounds must contain double bonds). The system can also be operated with a Mass Spectrometric (MS) detector; in this particular case the system is also equipped with an ion trap MS and with electrospray (ESI), Atmospheric Pressure Chemical ionization (APCI) and photoionization sources. These provide multiple ionization modes for the analysis of wide variety of compounds. Mass spectrometry is one of the most powerfull tools for obtaining molecular weights information and also in the assistance of structure elucidations. Caution, samples must be filtered using a 0.45µm filters before injecting into the system, otherwise it will clog the lines and columns increasing the pressure causing system failure. The system must be rinsed with water and methanol to remove salts from buffers before turning the system off. Please refer to Good Laboratory Practice * for more important information. Please refer to safety rules for information on how to manage samples.I will comply with the laboratory safety rules and general laboratory procedures. I agree to the terms of this page (Click Here)
* https://www2.unine.ch/files/content/sites/saf/files/shared/documents/GoodLaboratoryPractice-HPLC.pdf
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